A 96-well culture plate is a flat plastic plate with 96 small wells arranged in 8 rows and 12 columns. Each well can hold a small volume of culture medium. Scientists use this plate to grow and study cells under controlled conditions. This guide explains how to use a 96-well culture plate for cell growth experiments. All steps use simple and clear language for easy understanding. 1. Prepare the Work Area Before starting, clean your workspace. Use a laminar flow hood to keep the area sterile. Wear gloves and a lab coat. Disinfect tools and surfaces with 70% ethanol. Gather all needed items: culture media, cells, pipettes, sterile tips, and the 96-well plate. 2. Choose the Right 96-Well Culture Plate Select a plate suitable for cell growth. Material: Polystyrene (standard for cell culture). Surface treatment: Tissue culture-treated plates help cells attach and grow. Well format: 8 rows × 12 columns. Volume per well: 100-300 µL. Lid: Use a lid to prevent contamination and evaporation.
